Complementing the proteomics service in the Biochemistry Department, the Kavli INsD houses a wide range of mass spectrometers dedicated for collaborative projects in nanoscience. Using ‘soft’ ionisation methods and adhering to solution conditions that retain the folded state of the protein, a mass spectrum of a folded protein can be recorded. Such spectra provide insights into complex interactions including protein-protein contacts, lipid or ligand binding as well as antibody conjugation. Coupled with high resolution and highly sensitive detectors, this allows for accurate determination of mass, stoichiometry and, in some cases, small molecule/lipid binding partners.
Further opportunities exist to explore the overall topology of protein complexes in the gas phase via ion mobility measurements and through solution phase labelling techniques, including HDX.
For access, please contact Dr Andrew Dolan, the laboratory manager, or visit the Oxford Mass Spectrometry Centre Instruct-ERIC website (for pan-European, fully funded access).