Dr Daniel Zachor-Movshovitz - Chemistry
Revealing Red Blood Cells Extracellular Vesicles contents via Mass Photometry
Extracellular vesicles, EVs, are regularly secreted from cells carrying different cargo – i.e., proteins, DNA, RNA, etc., previous study shows red blood cells infected with malaria parasite secreted EVs (RBCEVs) contain proteins that help the growth of the parasite, one in particular is proteasome, weakening the cytoskeleton of an uninfected red blood cell allowing the parasite to infect other RBCs more easily. The current data gives a hint of the 20S, core particle of the proteasome and maybe a regulatory unit - - and we want to use Mass photometry to examine the EVs cargo and see which type of proteasome is it and thus learn of its function.
Why Mass photometry? because it’s easy to use and have a number of biological applications using solid lipid bilayers, membranes. I am trying to either cause leakage of the cargo in the EVs or cause the EVs to fuse to a solid lipid bilayer so the cargo will be released into the solution and measured by mass photometry. Important note is what is mass photometry – a light microscope designed to measure small changes in reflectance of a surface, in this case glass surface on top of which a protein of certain mass binds to. In doing so it reflects light differently than the glass itself and these two beams, the interference between them is measured (by a camera) and is proportional to the density and mass of the protein. Scan range is 30kDa to even mega Daltons. Easy to use and fast distribution of masses readout.
#RBCEVs, #Proteasome, #MassPhotometry, #MalariaParasite